ProtASR2: Ancestral reconstruction of protein sequences accounting for folding stability

The ancestral sequence reconstruction (ASR) is a molecular evolution technique that provides applications to a variety of fields such as biotechnology and biomedicine. To infer ancestral sequences with realistic biological properties, the accuracy of ASR methods is crucial. We previously developed an ASR framework for proteins, called ProtASR , which is based on our site‐specific stability‐constrained substitution (SCS) model with selection on...