The necessity of walnut proteolysis based on evaluation after in vitro simulated digestion: ACE inhibition and DPPH radical-scavenging activities

This study aimed to evaluate the necessity of enzymatic hydrolysis for walnut peptide preparation based on a novel evaluation approach. Defatted walnut meal hydrolysate (DWMH) was prepared by hydrolyzing defatted walnut meal (DWM) with alcalase, and gastrointestinal digestion of DWM and DWMH was simulated in vitro using pepsin and pancreatin. The peptide and free amino acid (FAA) contents, angiotensin-I-converting enzyme (ACE) inhibitory...