Investigation of growth kinetics and partial denitrification performance in strain Acinetobacter johnsonii under different environmental conditions

A denitrifying strain ZY04 with a high nitrite-accumulating rate was isolated and purified from activated sludge in a laboratory-scale A2/O reactor. The strain was characterized and identified as Acinetobacter johnsonii by 16S rDNA phylogenetic analysis. The sequences of the key functional genes (napA, nirB, nirD) involved in partial denitrification were amplified via polymerase chain reaction, which provided a basis for exploring gene...