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Short Communication: Failures in Detecting HTLV-1 and HTLV-2 in Patients Infected with HIV-1

Published on Apr 1, 2017in AIDS Research and Human Retroviruses1.805
· DOI :10.1089/aid.2016.0191
Karoline Rodrigues Campos2
Estimated H-index: 2
,
Maria Gisele Gonçalves6
Estimated H-index: 6
,
Adele Caterino-de-Araujo9
Estimated H-index: 9
Abstract
  • References (12)
  • Citations (3)
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1 Citations
1997
1 Author (良一 永富)
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References12
Newest
#1Camila CánepaH-Index: 4
#2Jimena SalidoH-Index: 3
Last. Mirna M. Biglione (UBA: University of Buenos Aires)H-Index: 13
view all 7 authors...
Background: indeterminate Western blot (WB) patterns are a major concern for diagnosis of human T-cell lymphotropic virus type 1 (HTLV-1) infection, even in non-endemic areas. Objectives: (a) to define the prevalence of indeterminate WB among different populations from Argentina; (b) to evaluate if low proviral load (PVL) is associated with indeterminate WB profiles; and (c) to describe mutations in LTR and tax sequence of these cases. Results: Among 2031 samples, 294 were reactive by screening....
7 CitationsSource
Abstract During the 1990s, high prevalences of HIV/human T lymphotropic virus type 1 (HTLV-1) and HIV/human T lymphotropic virus type 2 (HTLV-2) coinfections were detected in Sao Paulo, Brazil in association with intravenous drug use (IDU). The current prevalences and risk factors for HIV/HTLV-1/-2 were evaluated in 1,608 patients attending the AIDS/STD Reference and Training Center in Sao Paulo. Blood samples were analyzed for HTLV-1/2-specific antibodies using enzyme immunoassays (EIA Murex HT...
11 CitationsSource
#1Arthur Paiva (USP: University of São Paulo)H-Index: 5
#2Jorge Casseb (USP: University of São Paulo)H-Index: 18
Human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in many parts of the world and is primarily transmitted through sexual intercourse or from mother to child. Sexual transmission occurs more efficiently from men to women than women to men and might be enhanced by sexually transmitted diseases that cause ulcers and result in mucosal ruptures, such as syphilis, herpes simplex type 2 (HSV-2), and chancroid. Other sexually transmitted diseases might result in the recruitment of inflammatory ...
19 CitationsSource
Abstract Retroviral coinfections with HIV-1 and HTLV-1 or with HIV-1 and HTLV-2 occur with variable frequencies throughout the world with the highest prevalence in large metropolitan areas in the Americas, Europe, and Africa. The recognition that retroviral coinfections exist dates back to the discovery of HIV-1 over 25 years ago. Despite the large body of published information regarding the biological and clinical significance of retroviral coinfections, controversy throughout several decades o...
31 CitationsSource
#1Emanuela Avelar Silva Costa (Instituto Adolfo Lutz)H-Index: 3
#2Mariana Cavalheiro Magri (Instituto Adolfo Lutz)H-Index: 7
Last. Adele Caterino-de-Araujo (Instituto Adolfo Lutz)H-Index: 9
view all 3 authors...
Abstract The ability to confirm the diagnosis of human T-lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) in at-risk individuals in Sao Paulo, Brazil by Western blotting (WB), conventional polymerase chain reaction (tax and pol PCR) and real-time PCR (pol) is compared. Seventy-three blood samples that were reactive in HTLV-1/2 serological screening enzyme immunoassays (EIAs) were evaluated. HTLV-1/2 was confirmed in 53 blood samples: 48 were positive by WB, 41 were positive by PCR and 42 sco...
18 CitationsSource
#1Fabrício Jacob (Instituto Adolfo Lutz)H-Index: 3
#2Mariana Cavalheiro Magri (Instituto Adolfo Lutz)H-Index: 7
Last. Adele Caterino-de-Araujo (Instituto Adolfo Lutz)H-Index: 9
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Abstract Data obtained during routine diagnosis of human T-cell lymphotropic virus type 1 (HTLV-1) and 2 (HTLV-2) in “at-risk” individuals from Sao Paulo, Brazil using signal-to-cutoff (S/C) values obtained by first, second, and third generation enzyme immunoassay (EIA) kits, were compared. The highest S/C values were obtained with third generation EIA kits, but no correlation was detected between these values and specific antibody reactivity to HTLV-1, HTLV-2, or untyped HTLV (p = 0.302). In ad...
7 CitationsSource
#1Fabrício Jacob (Instituto Adolfo Lutz)H-Index: 3
#2Elizabeth de los Santos-Fortuna (Instituto Adolfo Lutz)H-Index: 8
Last. Adele Caterino-de-Araujo (Instituto Adolfo Lutz)H-Index: 9
view all 4 authors...
Abstract Background HTLV-1/2 diagnosis in high-risk populations from Sao Paulo, Brazil has been problematic due a high proportion of seroindeterminate results. Objectives To confirm and extend previous findings regarding HTLV-1/2 diagnosis in this geographic area. Study design Sera from 2312 patients were tested for HTLV-1/2 antibodies using enzyme immunoassay (EIA) and Western blot (WB) analysis. Patients were from AIDS Reference Centers (Group I; 1393 patients) and HTLV out-patient clinics (Gr...
16 CitationsSource
#1Helena Kaminami Morimoto (UEL: Universidade Estadual de Londrina)H-Index: 23
#2Arilson Akira Morimoto (UEL: Universidade Estadual de Londrina)H-Index: 7
Last. Adele Caterino-de-Araujo (Instituto Adolfo Lutz)H-Index: 9
view all 7 authors...
O diagnostico de infeccao por HTLV-2 se baseia na pesquisa de anticorpos especificos, entretanto, varios estudos conduzidos no Brasil tem apontado falhas nos kits sorologicos disponiveis no mercado em detectar HTLV-2, principalmente nos pacientes com HIV/aids. Este trabalho avaliou a presenca de infeccao por HTLV-1 e -2 em 758 pacientes HIV/aids de Londrina, Parana, Brasil. Amostras de soro foram analisadas quanto a presenca de anticorpos anti-HTLV-1/2 por dois kits de EIA (Vironostika e Murex) ...
19 CitationsSource
HTLV-I and HTLV-II infect T lymphocytes. A high HTLV-I proviral load in peripheral blood mononuclear cells (PBMCs) has been associated with a higher risk of neurologic disease. For HTLV-II, large numbers of infected lymphocytes might contribute to accelerate the immunodeficiency and increase the risk of neuropathy in HTLV-II/HIV-I coinfected people. We have examined the impact of antiretroviral drugs on HTLV proviral load, testing longitudinal samples collected from I HTLV-I infected patient suf...
39 CitationsSource
#1Adele Caterino-de-Araujo (UNIPD: University of Padua)H-Index: 8
#2Elizabeth de los Santos-Fortuna (Instituto Adolfo Lutz)H-Index: 8
Last. Luigi Chieco-Bianchi (UNIPD: University of Padua)H-Index: 39
view all 8 authors...
Abstract We investigated the presence of human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II) infections, first searching for specific antibodies in 553 serum samples obtained from HIV-1-infected patients from Sao Paulo, Brazil. Sera were screened using two enzyme-linked immunosorbent assays (ELISAs): the ELISA-EM (ELISA HTLV-I/II, EMBRABIO, BR), which contains HTLV-I and HTLV-II lysates, and the ELISA-DB [ELISA HTLV-I/II, Diagnostic Biotechnology (DB), Singapore], which contains ...
35 CitationsSource
Cited By3
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1 CitationsSource
#2Fred Luciano Neves Santos (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 7
Last. Adele Caterino-de-AraujoH-Index: 9
view all 7 authors...
Difficulties to confirm and discriminate human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) infections by serological Western Blotting (WB) assay (HTLV Blot 2.4, MP Biomedicals) has been reported in Brazil, mainly in HIV/AIDS patients, with a large number of WB-indeterminate and WB-positive but HTLV untypeable results. Nonetheless, the line immunoassay (LIA) (INNO-LIA HTLV-I/II, Fujirebio) was pointed to enhance specificity and sensitivity for confirming HTLV-1/2 infections. To ad...
1 CitationsSource