Enzymatic method and its validation for the micromolar assay of glucose in human serum samples
Abstract
This work depicts the novel chromogenic system for the assay of glucose in blood samples connected with the chelate formation of sulfosalicylic acid (SSA) with iron (Fe(III)) using glucose oxidase (GOD) presented. The purple-colored Fe-SSA chelate compound formed, maintain a strong absorption at 500 nm in the acidic buffer of pH 3.8. The Beer's law limit for the assay of glucose by rate, and a one-time method is in the range of 46–1295 μmol/L...
Paper Details
Title
Enzymatic method and its validation for the micromolar assay of glucose in human serum samples
Published Date
Feb 1, 2020
Journal
Volume
590
Pages
113536 - 113536
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