Crystal structures of the GH6Orpinomycessp. Y102 CelC7 enzyme with exo and endo activity and its complex with cellobiose

The catalytic domain (residues 128–449) of the Orpinomyces sp. Y102 CelC7 enzyme ( Orp CelC7) exhibits cellobiohydrolase and cellotriohydrolase activities. Crystal structures of Orp CelC7 and its cellobiose-bound complex have been solved at resolutions of 1.80 and 2.78 Å, respectively. Cellobiose occupies subsites +1 and +2 within the active site of Orp CelC7 and forms hydrogen bonds to two key residues: Asp248 and Asp409. Furthermore, its...