O- and N-glycosylation analysis of cell lines by ultrahigh resolution MALDI-FTICR-MS

Glycosylation analysis from biological samples is often challenging due to the high complexity of the glycan structures found in these samples. In the present study N- and O- glycans from human colorectal cancer cell lines and human plasma were analyzed using ultrahigh resolution MALDI-FTICR-MS. N-glycans were enzymatically released from cell lines and plasma proteins, whereas beta-elimination was used for the release of O-glycans from the...