Measuring the contribution of γδ T cells to the persistent HIV reservoir

Objective: To study the contribution of γδ T cells to the persistent HIV reservoir. Design: Fifteen HIV-seropositive individuals on suppressive ART were included. We performed parallel quantitative viral outgrowth assays (QVOA) of resting CD4 + T (rCD4) cells in the presence or absence of γδ T cells. Methods: Resting αβ+CD4 + T cells were magnetically isolated from PBMCs using two different custom cocktails, only one kit contained antibodies to...