Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a

Published: Feb 1, 2020
Abstract
Quantifying DNA cleavage by CRISPR-Cas nucleases is usually done by separating the cleaved products from the non-cleaved target by agarose gel electrophoresis. We devised a method that eliminates the quantification from band intensity on agarose gel, and uses a target with a fluorescent dye on the one end and a biotin on the other. Cleavage of the target will separate the dye from the biotin, and cause the dye to stay in solution when...
Paper Details
Title
Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a
Published Date
Feb 1, 2020
Journal
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