Graphene oxide and enzyme-assisted dual-cycling amplification method for sensitive fluorometric determination of DNA
Abstract
A fluorometric method is described for the determination of DNA. It involves the use of graphene oxide (GO), exonuclease III (Exo III), and two specially designed fluorophore-labeled hairpin probes (HP1 and HP2). Different from other GO-based DNA assays, the method takes advantage of the distinct binding abilities of GO with hairpin DNA probes and single nucleotides. GO serves as a strong quencher for fluorescent labels to ensure a very low...
Paper Details
Title
Graphene oxide and enzyme-assisted dual-cycling amplification method for sensitive fluorometric determination of DNA
Published Date
Oct 25, 2019
Journal
Volume
186
Issue
11
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