Biological plasticity rescues target activity in CRISPR knock outs
Abstract
Gene knock outs (KOs) are efficiently engineered through CRISPR-Cas9-induced frameshift mutations. While the efficiency of DNA editing is readily verified by DNA sequencing, a systematic understanding of the efficiency of protein elimination has been lacking. Here we devised an experimental strategy combining RNA sequencing and triple-stage mass spectrometry to characterize 193 genetically verified deletions targeting 136 distinct genes...
Paper Details
Title
Biological plasticity rescues target activity in CRISPR knock outs
Published Date
Oct 28, 2019
Journal
Volume
16
Issue
11
Pages
1087 - 1093
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Notes
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