pH profiles of cellulases depend on the substrate and architecture of the binding region

Understanding the pH effect of cellulolytic enzymes is of great technological importance. In this study, we have examined the influence of pH on activity and stability for central cellulases (Cel7A, Cel7B, Cel6A from Trichoderma reesei , and Cel7A from Rasamsonia emersonii ). We systematically changed pH from 2 to 7, temperature from 20°C to 70°C, and used both soluble (4‐nitrophenyl β‐ d ‐lactopyranoside [pNPL]) and insoluble (Avicel)...