Original paper
Guidelines for C to T base editing in plants: base-editing window, guide RNA length, and efficient promoter
Abstract
The Cas9 protein fused with a cytidine deaminase can induce C to T substitutions at a specific site when directed by a guide RNA. Here, we compared the substitution activity and the substitution range of two base-editing systems, APOBEC1-nCas9 and nCas9-PmCDA1, in the protoplasts of Glycine max, Brassica napus, and Nicotiana tabacum. To prevent unwanted nucleotide substitution, we manipulated the length of guide RNA and found the change of...
Paper Details
Title
Guidelines for C to T base editing in plants: base-editing window, guide RNA length, and efficient promoter
Published Date
Oct 1, 2019
Journal
Volume
13
Issue
5
Pages
533 - 541
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Notes
History