In vitro digestion method to evaluate solubility of dietary zinc, selenium and manganese in salmonid diets

The determination of dietary mineral solubility is one of the main steps in the evaluation of their availability for a given species.This study proposed an in vitro digestion method (acidic and alkaline hydrolysis). The method was applied to evaluate the solubility of inorganic and organic forms of zinc (Zn), selenium (Se) and manganese (Mn) in salmonid diets. An inorganic mineral (IM) diet was supplemented with zinc sulphate, sodium selenite...