Construction of full-length infectious cDNA clones of Apple stem grooving virus using Gibson Assembly method
Abstract
Apple stem grooving virus (ASGV) belongs to the genus Capillovirus within the family Betaflexiviridae. In this work, we described the construction of full-length infectious cDNA clones of ASGV isolate jilin-shaguo (JL-SG) using the Gibson Assembly approach (New England BioLabs). The isolate was previously detected in a Chinese pear-leaf crab apple (Malus asiatica Nakai.) in Baicheng, Jilin province, China. Two full-length cDNA clones of ASGV...
Paper Details
Title
Construction of full-length infectious cDNA clones of Apple stem grooving virus using Gibson Assembly method
Published Date
Jan 1, 2020
Journal
Volume
276
Pages
197790 - 197790
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