Search-and-replace genome editing without double-strand breaks or donor DNA
Abstract
Most genetic variants that contribute to disease1 are challenging to correct efficiently and without excess byproducts2-5. Here we describe prime editing, a versatile and precise genome editing method that directly writes new genetic information into a specified DNA site using a catalytically impaired Cas9 endonuclease fused to an engineered reverse transcriptase, programmed with a prime editing guide RNA (pegRNA) that both specifies the target...
Paper Details
Title
Search-and-replace genome editing without double-strand breaks or donor DNA
Published Date
Oct 21, 2019
Journal
Volume
576
Issue
7785
Pages
149 - 157
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