Free fatty acids promote transformation efficiency of yeast
Abstract
High transformation efficiency is essential in genetic engineering for functional metabolic analysis and cell factory construction, in particular in construction of long biosynthetic pathways with multiple genes. Here, we found that free fatty acid (FFA)-overproducing strain showed higher transformation efficiency in Saccharomyces cerevisiae. We then verified that external supplementation of FFAs, to the culture media for competent cell...
Paper Details
Title
Free fatty acids promote transformation efficiency of yeast
Published Date
Oct 9, 2019
Journal
Volume
19
Issue
7
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