Effective lysis of model thrombi by a t-PA mutant (A473S) that is resistant to α₂-antiplasmin

This study used two mutants of tissue‐type plasminogen activator (t‐PA) with resistance to inhibitors of fibrinolysis to define the contribution of plasminogen activator inhibitor (PAI)‐1 and α 2 ‐antiplasmin (α 2 ‐AP) to the control of fibrin lysis. Wild‐type t‐PA was compared with KHRR296–299AAAA, which is resistant to PAI‐1, and with A473S, which is resistant to α 2 ‐AP. We examined these forms of t‐PA in model systems that are...