Quantitative determination of cellular [Na+] by fluorescence lifetime imaging with CoroNaGreen
Abstract
Fluorescence lifetime imaging microscopy (FLIM) with fluorescent ion sensors enables the measurement of ion concentrations based on the detection of photon emission events after brief excitation with a pulsed laser source. In contrast to intensity-based imaging, it is independent of dye concentration, photobleaching, or focus drift and has thus been successfully employed for quantitative analysis of, e.g., calcium levels in different cell types...
Paper Details
Title
Quantitative determination of cellular [Na+] by fluorescence lifetime imaging with CoroNaGreen
Published Date
Oct 9, 2019
Volume
151
Issue
11
Pages
1319 - 1331
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