Improved library preparation with the new iCLIP2 protocol
Abstract
Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) is a state-of-the-art technology to map the RNA interaction sites of an RNA-binding protein (RBP) across the transcriptome. Here, we present the new iCLIP2 protocol that allows to obtain high-quality iCLIP libraries in a fast and efficient manner. The new protocol comprises separate adapter ligations, two cDNA amplification steps and bead-based size selection. The...
Paper Details
Title
Improved library preparation with the new iCLIP2 protocol
Published Date
Jun 1, 2020
Journal
Volume
178
Pages
33 - 48
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