Preparation and characterization of PDA/SiO2 nanofilm constructed macroporous monolith and its application in lipase immobilization

A monolithic macroporous silica (MMS) was prepared by using a pillared epoxy resin macroporous polymer as template. Polydopamine (PDA) as a spacer-arm polymer was modified on the surface of MMS for immobilization of Candida rugosa lipase (CRL), aiming to improve the performance of CRL and overcome the limitations of the immobilized enzymes on mesoporous materials and fine particles. The immobilization conditions and the properties of the...