Multi-spectroscopic and molecular docking technique study of the azelastine interaction with human serum albumin

Fluorescence and circular dichroism spectroscopic techniques and molecular docking were used to study binding of azelastine with human serum albumin (HSA). Time resolve fluorescence spectroscopy results indicated that the quenching mechanism is dynamic. Fluorescence quenching results demonstrated that the binding of azelastine to HSA is weak, binding reaction is spontaneous. There is fluorescence energy transfer from tryptophan of HSA to bound...