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An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids

Published on Sep 27, 2019in Molecules3.06
· DOI :10.3390/molecules24193516
Jing Li1
Estimated H-index: 1
,
Xianqing He1
Estimated H-index: 1
+ 1 AuthorsChenxi Yang1
Estimated H-index: 1
Abstract
Extracellular vesicles (EVs) are lipid bilayer enclosed particles which present in almost all types of biofluids and contain specific proteins, lipids, and RNA. Increasing evidence has demonstrated the tremendous clinical potential of EVs as diagnostic and therapeutic tools, especially in biofluids, since they can be detected without invasive surgery. With the advanced mass spectrometry (MS), it is possible to decipher the protein content of EVs under different physiological and pathological conditions. Therefore, MS-based EV proteomic studies have grown rapidly in the past decade for biomarker discovery. This review focuses on the studies that isolate EVs from different biofluids and contain MS-based proteomic analysis. Literature published in the past decade (2009.1–2019.7) were selected and summarized with emphasis on isolation methods of EVs and MS analysis strategies, with the aim to give an overview of MS-based EV proteomic studies and provide a reference for future research.
  • References (106)
  • Citations (2)
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References106
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#1Kaloyan Takov (UCL: University College London)H-Index: 4
#2Derek M. Yellon (UCL: University College London)H-Index: 100
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ABSTRACTInterest in small extracellular vesicles (sEVs) as functional carriers of proteins and nucleic acids is growing continuously. There are large numbers of sEVs in the blood, but lack of stand...
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#1T Arab (university of lille)H-Index: 1
#2Antonella Raffo-Romero (university of lille)H-Index: 3
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#1Yu Lin (SJTU: Shanghai Jiao Tong University)H-Index: 1
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Background Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-secretory products are necessary for downstream studies and application development. The most widely used purification method of EVs for helminth pathogens is currently differential centrifugation (DC). In contrast, size exclusion chromatography (SEC) has been included in the purification pipeline for EVs from other pathogens, highlighting there is not an agreed research community ‘gold...
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