Genome Engineering Renal Epithelial Cells for Enhanced Volume Transport Function

Bioengineering an implantable artificial kidney (IAK) will require renal epithelial cells capable of reabsorption of salt and water. We used genome engineering to modify cells for improved Na+/H+ exchange and H2O reabsorption. The non-viral piggyBac transposon system enables genome engineering cells to stably overexpress one or more transgenes simultaneously.We generated epitope-tagged human sodium hydrogen exchanger 3 (NHE3) and aquaporin-1...