Anti-Inflammatory Activity of the Wild Mushroom, Echinodontium tinctorium, in RAW264.7 Macrophage Cells and Mouse Microcirculation

Published on 2019in Molecules3.06
· DOI :10.3390/molecules24193509
Sumreen Javed1
Estimated H-index: 1
Wai Ming Li1
Estimated H-index: 1
+ 7 AuthorsChow H. Lee9
Estimated H-index: 9
The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, Echinodontium tinctorium, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract demonstrated potent anti-inflammatory activity, and was further purified using a “bio-activity-guided-purification” approach. The size-exclusion and ion-exchange chromatography yielded a water-soluble anti-inflammatory polysaccharide (AIPetinc). AIPetinc has an average molecular weight of 5 kDa, and is a heteroglucan composed of mainly glucose (88.6%) with a small amount of galactose (4.0%), mannose (4.4%), fucose (0.7%), and xylose (2.3%). In in vivo settings, AIPetinc restored the histamine-induced inflammatory event in mouse gluteus maximus muscle, thus confirming its anti-inflammatory activity in an animal model. This study constitutes the first report on the bioactivity of Echinodontium tinctorium, and highlights the potential medicinal benefits of fungi from the wild forests of northern British Columbia. Furthermore, it also reiterates the need to explore natural resources for alternative treatment to modern world diseases.
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#1Bożena Muszyńska (Jagiellonian University Medical College)H-Index: 12
#2Agata Grzywacz-Kisielewska (Jagiellonian University Medical College)H-Index: 1
Last.Joanna Gdula-Argasińska (Jagiellonian University Medical College)H-Index: 8
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#1Ankush Barad (UNBC: University of Northern British Columbia)H-Index: 1
#2Sebastian Mackedenski (UNBC: University of Northern British Columbia)H-Index: 3
Last.Kerry B. Reimer (UNBC: University of Northern British Columbia)H-Index: 5
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#1Shi-Liang Liu (BFU: Beijing Forestry University)H-Index: 1
#2Yan Zhao (BFU: Beijing Forestry University)H-Index: 1
Last.Shuang-Hui He (BFU: Beijing Forestry University)H-Index: 7
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#1Aaron Smith (UNBC: University of Northern British Columbia)H-Index: 1
#2Sumreen Javed (UNBC: University of Northern British Columbia)H-Index: 1
Last.Chow H. Lee (UNBC: University of Northern British Columbia)H-Index: 1
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#1Regan E Sevinsky (ETSU: East Tennessee State University)H-Index: 1
#2David W. Stewart (ETSU: East Tennessee State University)H-Index: 7
Last.Sam Harirforoosh (ETSU: East Tennessee State University)H-Index: 11
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#1Bin Du (Hebei Normal University of Science and Technology)H-Index: 13
#2Chengyuan Lin (Hong Kong Baptist University)H-Index: 13
Last.Baojun Xu (Hong Kong Baptist University)H-Index: 29
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