Anti-Inflammatory Activity of the Wild Mushroom, Echinodontium tinctorium, in RAW264.7 Macrophage Cells and Mouse Microcirculation.

Published on Sep 27, 2019in Molecules3.06
· DOI :10.3390/molecules24193509
Sumreen Javed1
Estimated H-index: 1
Wai Ming Li2
Estimated H-index: 2
+ 7 AuthorsChow H. Lee9
Estimated H-index: 9
The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, Echinodontium tinctorium, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract demonstrated potent anti-inflammatory activity, and was further purified using a “bio-activity-guided-purification” approach. The size-exclusion and ion-exchange chromatography yielded a water-soluble anti-inflammatory polysaccharide (AIPetinc). AIPetinc has an average molecular weight of 5 kDa, and is a heteroglucan composed of mainly glucose (88.6%) with a small amount of galactose (4.0%), mannose (4.4%), fucose (0.7%), and xylose (2.3%). In in vivo settings, AIPetinc restored the histamine-induced inflammatory event in mouse gluteus maximus muscle, thus confirming its anti-inflammatory activity in an animal model. This study constitutes the first report on the bioactivity of Echinodontium tinctorium, and highlights the potential medicinal benefits of fungi from the wild forests of northern British Columbia. Furthermore, it also reiterates the need to explore natural resources for alternative treatment to modern world diseases.
  • References (26)
  • Citations (1)
📖 Papers frequently viewed together
8 Citations
21 Citations
3 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Sumreen JavedH-Index: 1
#2Kevin J. MitchellH-Index: 29
Last. Geoffrey W. PayneH-Index: 11
view all 9 authors...
2 CitationsSource
#1Bożena Muszyńska (Jagiellonian University Medical College)H-Index: 14
#2Agata Grzywacz-Kisielewska (Jagiellonian University Medical College)H-Index: 1
Last. Joanna Gdula-Argasińska (Jagiellonian University Medical College)H-Index: 11
view all 4 authors...
Abstract Mushrooms have been used extensively, owing to their nutritional and medicinal value, for thousands of years. Modern research confirms the therapeutic effect of traditionally used species. Inflammation is a natural response of the immune system to damaging factors, e.g. physical, chemical and pathogenic. Deficiencies of antioxidants, vitamins, and microelements, as well as physiological processes, such as aging, can affect the body’s ability to resolve inflammation. Mushrooms are rich i...
38 CitationsSource
#1Ankush Barad (UNBC: University of Northern British Columbia)H-Index: 2
#2Sebastian Mackedenski (UNBC: University of Northern British Columbia)H-Index: 4
Last. Chow H. Lee (UNBC: University of Northern British Columbia)H-Index: 9
view all 12 authors...
• Polysaccharide (GIPinv) with anti-cancer activity was purified from Paxillus involutus .
4 CitationsSource
#1Yon-Suk Kim (Konkuk University)H-Index: 16
#2Woen-Bin Shin (Konkuk University)H-Index: 3
Last. Pyo-Jam Park (Konkuk University)H-Index: 34
view all 7 authors...
In our previous work, Asterina pectinifera was fermented with Cordyceps militaris mycelia to improve its bioactivities and was reported to have strong antioxidant activities. The aim of the current study was to investigate its anti-inflammatory effect and mechanisms of action. In this study, we observed the inhibitory effect of the extract from fermented A. pectinifera with C. militaris mycelia (FACM) on nitric oxide (NO) production and its molecular mechanism in lipopolysaccharide (LPS)-stimula...
8 CitationsSource
#1Shi-Liang Liu (BFU: Beijing Forestry University)H-Index: 1
#2Yan Zhao (BFU: Beijing Forestry University)H-Index: 1
Last. Shuang-Hui He (BFU: Beijing Forestry University)H-Index: 7
view all 5 authors...
The phylogenetic relationship of eight species of Echinodontium, Laurilia, and Perplexostereum of Russulales were analyzed based on sequences of the nuc rDNA ITS1-5.8S-ITS2 (ITS [internal transcribed spacer]) and D1–D2 domains of nuc 28S rDNA (28S). Our results show that Echinodontium tinctorium, E. ryvardenii, and E. tsugicola represent Echinodontium sensu stricto. Based on morphological and phylogenetic evidence, the new genus Echinodontiellum is established to accommodate Echinodontium japoni...
3 CitationsSource
#1Regan E Sevinsky (ETSU: East Tennessee State University)H-Index: 1
#2David W. Stewart (ETSU: East Tennessee State University)H-Index: 8
Last. Sam Harirforoosh (ETSU: East Tennessee State University)H-Index: 12
view all 3 authors...
3 CitationsSource
#1Aaron Smith (UNBC: University of Northern British Columbia)H-Index: 1
#2Sumreen Javed (UNBC: University of Northern British Columbia)H-Index: 1
Last. Chow H. Lee (UNBC: University of Northern British Columbia)H-Index: 1
view all 11 authors...
3 CitationsSource
Abstract Background In the recent years natural resources are being in focus due to their great potential to be exploited in the discovery/development of novel bioactive compounds and, among them, mushrooms can be highlighted as alternative sources of anti-inflammatory agents. Scope and approach The present review reports the anti-inflammatory activity of mushroom extracts and of their bioactive metabolites involved in this bioactive action. Additionally the most common assays used to evaluate m...
31 CitationsSource
#1Christian Bogdan (FAU: University of Erlangen-Nuremberg)H-Index: 56
Thirty years after the discovery of its production by activated macrophages, our appreciation of the diverse roles of nitric oxide (NO) continues to grow. Recent findings have not only expanded our understanding of the mechanisms controlling the expression of NO synthases (NOS) in innate and adaptive immune cells, but have also revealed new functions and modes of action of NO in the control and escape of infectious pathogens, in T and B cell differentiation, and in tumor defense. I discuss these...
271 CitationsSource
Cited By1
#1Maria Graça Miguel (University of the Algarve)H-Index: 24
#1Chunyan Hou (Zhengzhou University of Light Industry)H-Index: 2
view all 4 authors...
Abstract Inflammation is the first response of the body's immune system to injury, infection and stress. Chronic inflammation has close relationship with many diseases, such as arthritis, atherosclerosis and cancer. The traditional therapy strategy of inflammation mainly involves steroid and non-steroid anti-inflammatory drugs, both of which have increasingly prominent side-effects which cannot be ignored. Polysaccharides extracted from natural sources have attracted extensive attention in recen...