Extracellular production of recombinant N-glycosylated anti-VEGFR2 monobody in leaky Escherichia coli strain
Abstract
To improve the production yield of N-glycosylated anti-VEGFR2 (vascular endothelial growth factor receptor 2) monobody (FN3VEGFR2-Gly) in lpp knockout Escherichia coli cells harboring Campylobacter jejuni N-glycosylation pathway. The leaky CLM37-Δlpp strain efficiently secreted FN3VEGFR2-Gly into culture medium. The extracellular levels of glycosylated FN3VEGFR2-Gly in CLM37-Δlpp culture medium were approximately 11 and 15 times higher compared...
Paper Details
Title
Extracellular production of recombinant N-glycosylated anti-VEGFR2 monobody in leaky Escherichia coli strain
Published Date
Sep 20, 2019
Journal
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