Molecular form and concentration of serum α2-macroglobulin in diabetes

α 2 -Macroglobulin is a highly abundant serum protein involved in the development of atherosclerosis and cardiac hypertrophy. However, its circulating molecular form and exact concentrations in human health/diseases are not known. Blue native-polyacrylamide gel electrophoresis of human serum was used to confirm the native conformation of α 2 -macroglobulin. We created an enzyme-linked immunosorbent assay suitable for quantifying its circulating...