Identification of a variant of CMS-T cytoplasm and development of high resolution melting markers for distinguishing cytoplasm types and genotyping a restorer-of-fertility locus in onion (Allium cepa L.)

Cytoplasmic male-sterility (CMS) has been used in F1 hybrid seed production in onion (Allium cepa L.). Two types of CMS (CMS-S and CMS-T) have been reported in onions. Almost complete mitochondrial genome sequences of normal, CMS-T, and CMS-S cytoplasm types have been obtained in previous studies. Unlike highly divergent CMS-S mitochondrial genome, there were only three single nucleotide polymorphisms (SNPs) between normal and CMS-T...