Functional flow cytometry of monocytes for routine diagnosis of innate primary immunodeficiencies

Flow cytometry is valuable for diagnosing primary immunodeficiencies (PIDs). In addition to analyzing the presence or absence of immune cells, it can provide functional information on, for example, protein expression or signaling pathways. This is useful for PID screening or for validating genetic variants of uncertain significance (VUS). Although numerous tests are available for the diagnosis of PIDs affecting adaptive immunity, clinically...