Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1
Abstract
CRISPR-associated (Cas) nucleases are established tools for engineering of animal genomes. These programmable RNA-guided nucleases have been introduced into zygotes using expression vectors, mRNA, or directly as ribonucleoprotein (RNP) complexes by different delivery methods. Whereas microinjection techniques are well established, more recently developed electroporation methods simplify RNP delivery but can provide less consistent efficiency....
Paper Details
Title
Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1
Published Date
Sep 3, 2019
Journal
Volume
28
Issue
5-6
Pages
525 - 535
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