Reduced fire blight susceptibility in apple cultivars using a high‐efficiency CRISPR/Cas9‐FLP/FRT‐based gene editing system

Summary The bacterium Erwinia amylovora , the causal agent of fire blight disease in apple, triggers its infection through the DspA/E effector which interacts with the apple susceptibility protein Md DIPM 4. In this work, Md DIPM 4 knockout has been produced in two Malus × domestica susceptible cultivars using the CRISPR /Cas9 system delivered via Agrobacterium tumefaciens . Fifty‐seven transgenic lines were screened to identify CRISPR...