Methods for analysis of circular RNAs

Eukaryotic cells express a myriad of circular RNAs (circRNAs), many of them displaying tissue‐specific expression patterns. They arise from linear precursor RNAs in which 5′ and 3′ ends become covalently ligated. Given these features, biochemical and computational approaches traditionally used to study linear RNA must be adapted for analysis of circular RNAs. Such circRNA‐specific methodologies are allowing the systematic identification of...