Simultaneous Determination of 35 Organic Metabolites Including Amino Acids, Creatine, Creatinine, and Oxidized and Reduced Glutathione in Lung Tissue, Plasma and Urine Using Liquid Chromatography–Tandem Mass Spectrometry

A simple and rapid method for the simultaneous determination of 35 non-derivatized metabolites was developed using liquid chromatography – tandem mass spectrometry. Under the optimal conditions, linearity was achieved in the range from 0.05 to 10.0 ng (R2 ≥ 0.989). The repeatability provided percentage relative standard deviations ≤11.2 and a trueness expressed as percentage relative error from −10.7% to 12%. The recoveries ranged from 87.3% to...