Immobilization of fungal inulinase on hetero-functionalized carbon nanofibers for the production of fructose from inulin

The present study describes the response surface optimization (RSM) of inulinase immobilization on carbon nanofibers (CNFs), modified using 3-aminopropyl-triethoxysilane (APTES) and glutaraldehyde (GA). Immobilized protein content and inulinase activity were used as indicators of immobilization. Under RSM optimised conditions viz., sonication time 120 min, APTES concentration 3.0%, GA concentration 2.0%, GA activation time 75 min, enzyme load...