Generation of a multiplex mutagenesis population via pooled CRISPR‐Cas9 in soya bean

Summary The output of genetic mutant screenings in soya bean [ Glycine max (L.) Merr.] has been limited by its paleopolypoid genome. CRISPR ‐Cas9 can generate multiplex mutants in crops with complex genomes. Nevertheless, the transformation efficiency of soya bean remains low and, hence, remains the major obstacle in the application of CRISPR ‐Cas9 as a mutant screening tool. Here, we report a pooled CRISPR ‐Cas9 platform to generate soya bean...