Using hapten cross-reactivity to screen heterologous competitive antigens for improving the sensitivity of ELISA

In this study, heterologous competitive antigens (HCAs) suitable for improving the sensitivity of ELISA were successfully screened based on their cross-reactivities (CRs) with 19 quinolone analogues; each containing the norfloxacin amino derivative (NOR0) coupled with bovine serum albumin (BSA) as a coating antigen. HCAs prepared with hapten analogues (CRs of 0.77%–49.92%) remarkably enhanced the sensitivity of the subsequent ELISA. ELISA...