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Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples

Published on 2019in Environment International7.94
· DOI :10.1016/j.envint.2019.105091
Lanhua Liu8
Estimated H-index: 8
(THU: Tsinghua University),
Xiaohong Zhou14
Estimated H-index: 14
(THU: Tsinghua University)
+ 3 AuthorsTong Yu2
Estimated H-index: 2
(U of A: University of Alberta)
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Abstract
Abstract Establishing biosensors to map a comprehensive picture of potential estrogen-active chemicals remains challenging and must be addressed. Herein, we describe an estrogen receptor (ER)-based evanescent wave fluorescent biosensor by using a triple functional small-molecule–protein conjugate as a signal probe for the determination of estrogenic activities in water samples. The signal probe, consisting of a Cy5.5-labelled streptavidin (STV) moiety and a 17β-estradiol (E2) moiety, acts simultaneously as signal conversion, signal recognition and signal report elements. When xenoestrogens compete with the E2 moiety of conjugate in binding to the ER, the unbound conjugates are released, and their STV moiety binds with desthiobiotin (DTB) modified on the optical fiber via the STV–DTB affinity interactions. Signal probe detection is accomplished by fluorescence emission induced by an evanescent field, which positively relates with the estrogenic activities in samples. Quantification of estrogenic activity expressed as E2 equivalent concentration (EEQ) can be achieved with a detection limit of 1.05 μg/L EEQ by using three times standard deviation of the mean blank values and a linear calibration range from 20.8 to 476.7 μg/L EEQ. The optical fiber system is robust enough for hundreds of sensing cycles. The biosensor-based determination of estrogenic activities in wastewater samples obtained from a full-scale wastewater treatment plant is consistent with that measured by the two-hybrid recombinant yeast bioassay.
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References44
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#1Lanhua Liu (THU: Tsinghua University)H-Index: 8
#2Xiaohong Zhou (THU: Tsinghua University)H-Index: 14
Last.Yi Qian (THU: Tsinghua University)H-Index: 31
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#1Justin M. Conley (ORISE: Oak Ridge Institute for Science and Education)H-Index: 1
#2Nicola Evans (EPA: United States Environmental Protection Agency)H-Index: 2
Last.Vickie S. Wilson (EPA: United States Environmental Protection Agency)H-Index: 41
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#1Muhammad Adeel (Shenyang University)H-Index: 10
#2Xiaoming Song (Shenyang University)H-Index: 2
Last.Yuesuo Yang (Shenyang University)H-Index: 12
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#1J. Porter Hunt (BYU: Brigham Young University)H-Index: 2
#2Song-Min Schinn (BYU: Brigham Young University)H-Index: 4
Last.Bradley C. Bundy (BYU: Brigham Young University)H-Index: 16
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#1Viviana Scognamiglio (National Research Council)H-Index: 19
#2Amina Antonacci (National Research Council)H-Index: 11
Last.Giuseppina Rea (National Research Council)H-Index: 20
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