High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins
Abstract
Single-molecule Förster resonance energy transfer (smFRET) is a powerful technique for nanometer-scale studies of single molecules. Solution-based smFRET, in particular, can be used to study equilibrium intra- and intermolecular conformations, binding/unbinding events and conformational changes under biologically relevant conditions without ensemble averaging. However, single-spot smFRET measurements in solution are slow. Here, we detail a...
Paper Details
Title
High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins
Published Date
Oct 1, 2019
Journal
Volume
169
Pages
21 - 45
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