Structure functional insights into calcium binding during the activation of coagulation factor XIII A

The dimeric FXIII-A 2 , a pro-transglutaminase is the catalytic part of the heterotetrameric coagulation FXIII-A 2 B 2 complex that upon activation by calcium binding/thrombin cleavage covalently cross-links preformed fibrin clots protecting them from premature fibrinolysis. Our study characterizes the recently disclosed three calcium binding sites of FXIII-A concerning evolution, mutual crosstalk, thermodynamic activation profile, substrate...