Improved strategy based on QuEChERS method followed by HPLC/DAD for the quantification of phenolic compounds from Mimosa scabrella Bentham honeydew honeys
Abstract Sample preparation of honey for analysis of minor components as phenolic compounds is challenging, mainly due to the high sugar content. In this regard, a fast, simple and solvent saving method for extraction of phenolic compounds in Mimosa scabrella Bentham honeydew honeys was proposed. The method was based on QuEChERS, and the extraction, salting-out and clean-up steps were studied through univariate experiments. Solvent volume, solvent pH, partition salts, and clean-up salts were the variables studied. The responses were considered the recovery of selected phenolic compounds and the visual inspection of the bracatinga honeydew honeys chromatograms. The final sample preparation used 4 mL of acetonitrile (with 1% formic acid) besides 1 g NaCl and 4 g MgSO4 on the extraction step. For the clean-up step, 150 mg CaCl2, 50 mg PSA, and 50 mg C18 were used. After validation according to the Eurachem guidelines, the proposed QuEChERS method was applied for sample preparation of 52 bracatinga honeydew honey samples. Sixteen compounds were quantified by HPLC/DAD and Principal Components Analysis was used to distinguish samples regarding their phenolic composition. QuEChERS was useful for the phenolic compounds extraction from bracatinga honeydew honey and could be successfully applied for its determination in other honeys.