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Steroids originating from bacterial bile acid degradation affect Caenorhabditis elegans and indicate potential risks for the fauna of manured soils

Published in Scientific Reports4.01
· DOI :10.1038/s41598-019-47476-y
Martha N. Mendelski1
Estimated H-index: 1
(WWU: University of Münster),
Ramona Dölling1
Estimated H-index: 1
(WWU: University of Münster)
+ -3 AuthorsBodo Philipp20
Estimated H-index: 20
(WWU: University of Münster)
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Abstract
Bile acids are steroid compounds from the digestive tracts of vertebrates that enter agricultural environments in unusual high amounts with manure. Bacteria degrading bile acids can readily be isolated from soils and waters including agricultural areas. Under laboratory conditions, these bacteria transiently release steroid compounds as degradation intermediates into the environment. These compounds include androstadienediones (ADDs), which are C19-steroids with potential hormonal effects. Experiments with Caenorhabditis elegans showed that ADDs derived from bacterial bile acid degradation had effects on its tactile response, reproduction rate, and developmental speed. Additional experiments with a deletion mutant as well as transcriptomic analyses indicated that these effects might be conveyed by the putative testosterone receptor NHR-69. Soil microcosms showed that the natural microflora of agricultural soil is readily induced for bile acid degradation accompanied by the transient release of steroid intermediates. Establishment of a model system with a Pseudomonas strain and C. elegans in sand microcosms indicated transient release of ADDs during the course of bile acid degradation and negative effects on the reproduction rate of the nematode. This proof-of-principle study points at bacterial degradation of manure-derived bile acids as a potential and so-far overlooked risk for invertebrates in agricultural soils.
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Published on Dec 1, 2019in Mbio6.75
H Armstrong3
Estimated H-index: 3
(U of A: University of Alberta),
Misagh Alipour3
Estimated H-index: 3
(U of A: University of Alberta)
+ 15 AuthorsAndrew L. Mason38
Estimated H-index: 38
(U of A: University of Alberta)
Inflammatory bowel diseases (IBD) are a group of complex and multifactorial disorders with unknown etiology. Chronic intestinal inflammation develops against resident intestinal bacteria in genetically susceptible hosts. We hypothesized that host intestinal immunoglobulin (Ig) G can be used to identify bacteria involved in IBD pathogenesis. IgG-bound and -unbound microorganisms were collected from 32 pediatric terminal ileum aspirate washes during colonoscopy [non-IBD (n = 10), Crohn disease (n ...
Published on Jun 8, 2018in Applied and Environmental Microbiology4.08
Baojun Wu7
Estimated H-index: 7
(Clark University),
Jill Gaskell21
Estimated H-index: 21
(USDA: United States Department of Agriculture)
+ 11 AuthorsEmma R. Master24
Estimated H-index: 24
(U of T: University of Toronto)
ABSTRACT Wood-decaying fungi tend to have characteristic substrate ranges that partly define their ecological niche. Fomitopsis pinicola is a brown rot species of Polyporales that is reported on 82 species of softwoods and 42 species of hardwoods. We analyzed the gene expression levels and RNA editing profiles of F. pinicola from submerged cultures with ground wood powder (sampled at 5 days) or solid wood wafers (sampled at 10 and 30 days), using aspen, pine, and spruce substrates (aspen was use...
Published on Jan 30, 2018in Mbio6.75
Johannes Holert9
Estimated H-index: 9
(UBC: University of British Columbia),
Erick Cardenas15
Estimated H-index: 15
(UBC: University of British Columbia)
+ 4 AuthorsWilliam W. Mohn51
Estimated H-index: 51
(UBC: University of British Columbia)
ABSTRACT Steroids are abundant growth substrates for bacteria in natural, engineered, and host-associated environments. This study analyzed the distribution of the aerobic 9,10-seco steroid degradation pathway in 346 publically available metagenomes from diverse environments. Our results show that steroid-degrading bacteria are globally distributed and prevalent in particular environments, such as wastewater treatment plants, soil, plant rhizospheres, and the marine environment, including marine...
Published on Dec 1, 2017in Molecular Genetics and Genomics2.88
Alex Keshet3
Estimated H-index: 3
(WWU: University of Münster),
Ansgar Mertenskötter4
Estimated H-index: 4
(WWU: University of Münster)
+ 3 AuthorsRüdiger J. Paul26
Estimated H-index: 26
(WWU: University of Münster)
The mechanisms of cadmium (Cd) resistance are complex and not sufficiently understood. The present study, therefore, aimed at assessing the roles of important components of stress-signaling pathways and of ABC transporters under severe Cd stress in Caenorhabditis elegans. Survival assays on mutant and control animals revealed a significant promotion of Cd resistance by the PMK-1 p38 MAP kinase, the transcription factor DAF-16/FoxO, and the ABC transporter MRP-1. Transcriptome profiling by RNA-Se...
Published on Nov 1, 2017in Environmental Microbiology5.15
Markus Warnke3
Estimated H-index: 3
,
Christian Jacoby2
Estimated H-index: 2
+ 8 AuthorsThomas Brüls13
Estimated H-index: 13
(Université Paris-Saclay)
Published on Oct 20, 2017in Applied and Environmental Microbiology4.08
Onur Yücel5
Estimated H-index: 5
,
Johannes Holert9
Estimated H-index: 9
(UBC: University of British Columbia)
+ 2 AuthorsBodo Philipp20
Estimated H-index: 20
ABSTRACT Bile salts such as cholate are steroid compounds with a C 5 carboxylic side chain and occur ubiquitously in vertebrates. Upon their excretion into soils and waters, bile salts can serve as growth substrates for diverse bacteria. Novosphingobium sp. strain Chol11 degrades 7-hydroxy bile salts via 3-keto-7-deoxy-Δ 4,6 metabolites by the dehydration of the 7-hydroxyl group catalyzed by the 7α-hydroxysteroid dehydratase Hsh2. This reaction has not been observed in the well-studied 9-10-seco...
Published on Oct 18, 2017in PLOS ONE2.78
Caspar A. Hallmann7
Estimated H-index: 7
(Radboud University Nijmegen),
Martin Sorg1
Estimated H-index: 1
+ 9 AuthorsThomas Hörren1
Estimated H-index: 1
Global declines in insects have sparked wide interest among scientists, politicians, and the general public. Loss of insect diversity and abundance is expected to provoke cascading effects on food webs and to jeopardize ecosystem services. Our understanding of the extent and underlying causes of this decline is based on the abundance of single species or taxonomic groups only, rather than changes in insect biomass which is more relevant for ecological functioning. Here, we used a standardized pr...
Published on May 3, 2017in Mbio6.75
Judith Straimer12
Estimated H-index: 12
,
Nina F. Gnädig10
Estimated H-index: 10
+ 3 AuthorsDavid A. Fidock63
Estimated H-index: 63
Published on May 3, 2017in Mbio6.75
Adam M. Crowe7
Estimated H-index: 7
(UBC: University of British Columbia),
Israël Casabon12
Estimated H-index: 12
(UBC: University of British Columbia)
+ 7 AuthorsLindsay D. Eltis49
Estimated H-index: 49
(UBC: University of British Columbia)
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