Adaptation and application of a two-plasmid inducible CRISPR-Cas9 system in Clostridium beijerinckii
Abstract
Recent developments in CRISPR technologies have opened new possibilities for improving genome editing tools dedicated to the Clostridium genus. In this study we adapted a two-plasmid tool based on this technology to enable scarless modification of the genome of two reference strains of Clostridium beijerinckii producing an Acetone/Butanol/Ethanol (ABE) or an Isopropanol/Butanol/Ethanol (IBE) mix of solvents. In the NCIMB 8052 ABE-producing...
Paper Details
Title
Adaptation and application of a two-plasmid inducible CRISPR-Cas9 system in Clostridium beijerinckii
Published Date
Feb 1, 2020
Journal
Volume
172
Pages
51 - 60
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