Purification of monoclonal antibodies in a stirred cell with polyethyleneimine‐modified polyethersulfone ultrafiltration membrane

BACKGROUND Charged ultrafiltration membranes combined with optimal operating conditions can be used to enable monoclonal antibody purification. Positively charged ligands bound to the membrane surface can significantly contribute to the adsorption of negatively charged proteins and enhance the purification process. RESULTS In this work, commercially available 500 kDa polyethersulfone ultrafiltration membranes were modified by sulfonation,...