Improving production of N-glycosylated recombinant proteins by leaky Escherichia coli
Abstract
Escherichia coli has been considered as a promising host for the production of N-glycosylated therapeutic proteins and glycoconjugate vaccines. In this study, we developed a simple and efficient strategy for improving the production of N-glycosylated recombinant proteins by combining auto-induction with the use of a leaky E. coli strain. A leaky E. coli strain, designated as CLM37-Δlpp, was engineered by deleting the Braun’s lipoprotein (lpp)...
Paper Details
Title
Improving production of N-glycosylated recombinant proteins by leaky Escherichia coli
Published Date
Jul 22, 2019
Journal
Volume
9
Issue
8
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