An effective FRET-based two-photon ratiometric fluorescent probe with double well-resolved emission bands for lysosomal pH changes in living cells and zebrafish

In cells, lysosome is an acidic organelle (approximately pH 4.5–5.5), whose pH changes plays a key role in mediating various biological processes. To address this issue, a lot of fluorescent probes have been developed and prepared for tracking lysosomal pH changes. However, few of these probes can realize the imaging of lysosomal pH changes in biosystems. Herein, a new two-photon (TP) ratiometric fluorescent probe (NpRhLys-pH) by adopting the...