The cysteine‐free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications
Abstract
APEX2, an engineered ascorbate peroxidase for high activity, is a powerful tool for proximity labeling applications. Owing to its lack of disulfides and the calcium-independent activity, APEX2 can be applied intracellularly for targeted electron microscopy imaging or interactome mapping when fusing to a protein of interest. However, APEX2 fusion is often deleterious to the protein expression, which seriously hampers its wide utility. This...
Paper Details
Title
The cysteine‐free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications
Published Date
Aug 6, 2019
Journal
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