VARSCOT: variant-aware detection and scoring enables sensitive and personalized off-target detection for CRISPR-Cas9

Natural variations in a genome can drastically alter the CRISPR-Cas9 off-target landscape by creating or removing sites. Despite the resulting potential side-effects from such unaccounted for sites, current off-target detection pipelines are not equipped to include variant information. To address this, we developed VARiant-aware detection and SCoring of Off-Targets (VARSCOT). VARSCOT identifies only 0.6% of off-targets to be common between 4...