Match!

Transcriptional reprogramming caused by the geminivirus Tomato yellow leaf curl virus in local or systemic infections in Nicotiana benthamiana

Published on Dec 1, 2019in BMC Genomics3.50
· DOI :10.1186/s12864-019-5842-7
Mengshi Wu (CAS: Chinese Academy of Sciences), Xue Ding1
Estimated H-index: 1
(CAS: Chinese Academy of Sciences)
+ 1 AuthorsRosa Lozano-Durán15
Estimated H-index: 15
(CAS: Chinese Academy of Sciences)
Cite
Abstract
Viruses have evolved to create a cellular environment permissive for viral replication in susceptible hosts. Possibly both enabling and resulting from these virus-triggered changes, infected hosts undergo a dramatic transcriptional reprogramming, the analysis of which can shed light on the molecular processes underlying the outcome of virus-host interactions. The study of the transcriptional changes triggered by the plant DNA viruses geminiviruses is potentially hampered by the low representation of infected cells in the total population, a situation that becomes extreme in those cases, like that of Tomato yellow leaf curl virus (TYLCV), in which the virus is restricted to phloem companion cells. In order to gain insight into how different the transcriptional landscapes of TYLCV-infected cells or whole tissues of TYLCV-infected plants might be, here we compare the transcriptional changes in leaf patches infected with TYLCV by agroinfiltration or in systemic leaves of TYLCV-infected plants in Nicotiana benthamiana. Our results show that, in agreement with previous works, infection by TYLCV induces a dramatic transcriptional reprogramming; the detected changes, however, are not equivalent in local and systemic infections, with a much larger number of genes differentially expressed locally, and some genes responding in an opposite manner. Interestingly, a transcriptional repression of the auxin signalling pathway and a transcriptional activation of the ethylene signalling pathway were detected in both local and systemically infected samples. A transcriptional activation of defence was also detectable in both cases. Comparison with the transcriptional changes induced by systemic infection by the geminivirus Tobacco curly shoot virus (TbSV) shows common subsets of up- and down-regulated genes similarly affected by both viral species, unveiling a common transcriptional repression of terpenoid biosynthesis, a process also suppressed by the geminivirus Tomato yellow leaf curl China virus. Taken together, the results presented here not only offer insight into the transcriptional changes derived from the infection by TYLCV in N. benthamiana, but also demonstrate that the resolution provided by local and systemic infection approaches largely differs, highlighting the urge to come up with a better system to gain an accurate view of the molecular and physiological changes caused by the viral invasion.
  • References (31)
  • Citations (0)
Cite
References31
Newest
Published on Dec 1, 2018in Virology Journal2.46
Ke Li2
Estimated H-index: 2
(SWU: Southwest University),
Gentu Wu1
Estimated H-index: 1
(SWU: Southwest University)
+ 5 AuthorsLing Qing5
Estimated H-index: 5
(SWU: Southwest University)
Background Tobacco curly shoot virus (TbCSV) is a monopartite begomovirus associated with betasatellite (Tobacco curly shoot betasatellite, TbCSB), which causes serious leaf curl disease on tomato and tobacco in China. It is interesting that TbCSV induced severe upward leaf curling in Nicotiana benthamiana, but in the presence of TbCSB, symptoms changed to be downward leaf curling. However, the mechanism of interactions between viral pathogenicity, host defense, viral-betasatellite interactions ...
Published on Mar 15, 2018in International Journal of Molecular Sciences4.18
Katarzyna Otulak-Kozieł1
Estimated H-index: 1
(Warsaw University of Life Sciences),
Edmund Kozieł2
Estimated H-index: 2
(Warsaw University of Life Sciences),
Ben Lockhart26
Estimated H-index: 26
The cell wall provides the structure of the plant, and also acts as a barier against biotic stress. The vein necrosis strain of Potato virus Y (PVYNTN) induces necrotic disease symptoms that affect both plant growth and yield. Virus infection triggers a number of inducible basal defense responses, including defense proteins, especially those involved in cell wall metabolism. This study investigates the comparison of cell wall host dynamics induced in a compatible (potato cv. Irys) and incompatib...
Published on Mar 1, 2018in Virology2.66
Jang-Kyun Seo12
Estimated H-index: 12
(SNU: Seoul National University),
Mi-Kyeong Kim6
Estimated H-index: 6
(RDA: Rural Development Administration)
+ 7 AuthorsJin-Ho Kang13
Estimated H-index: 13
(SNU: Seoul National University)
Abstract The viral infection of plants may cause various physiological symptoms associated with the reprogramming of plant gene expression. However, the molecular mechanisms and associated genes underlying disease symptom development in plants infected with viruses are largely unknown. In this study, we employed RNA sequencing for in-depth molecular characterization of the transcriptional changes associated with the development of distinct symptoms induced by tomato chlorosis virus (ToCV) and to...
Tábata Rosas-Díaz5
Estimated H-index: 5
(CAS: Chinese Academy of Sciences),
Dan Zhang2
Estimated H-index: 2
(CAS: Chinese Academy of Sciences)
+ 17 AuthorsZhengyan Feng7
Estimated H-index: 7
(CAS: Chinese Academy of Sciences)
RNA interference (RNAi) in plants can move from cell to cell, allowing for systemic spread of an antiviral immune response. How this cell-to-cell spread of silencing is regulated is currently unknown. Here, we describe that the C4 protein from Tomato yellow leaf curl virus can inhibit the intercellular spread of RNAi. Using this viral protein as a probe, we have identified the receptor-like kinase (RLK) BARELY ANY MERISTEM 1 (BAM1) as a positive regulator of the cell-to-cell movement of RNAi, an...
Published on Dec 22, 2017in Frontiers in Plant Science4.11
Liping Wang (CAS: Chinese Academy of Sciences), Huang Tan (CAS: Chinese Academy of Sciences)+ 3 AuthorsRosa Lozano-Durán15
Estimated H-index: 15
(CAS: Chinese Academy of Sciences)
Viruses are intracellular parasites with a nucleic acid genome and a proteinaceous capsid. Viral capsids are formed of at least one virus-encoded capsid protein (CP), which is often multifunctional, playing additional non-structural roles during the infection cycle. In animal viruses, there are examples of differential localization of CPs associated to the progression of the infection and/or enabled by other viral proteins; these changes in the distribution of CPs may ultimately regulate the inv...
Published on Feb 1, 2017in Plant Cell Reports3.50
Rong Lei3
Estimated H-index: 3
,
Hongshan Jiang3
Estimated H-index: 3
+ 2 AuthorsShuifang Zhu2
Estimated H-index: 2
Key message Leaf chlorosis induced by plant virus infection has a short fluorescence lifetime, which reflects damaged photosynthetic complexes and degraded chloroplasts.
Published on Dec 1, 2015in Virus Genes1.62
Bengyella Louis1
Estimated H-index: 1
(University of the Witwatersrand),
Chrissie Rey4
Estimated H-index: 4
(University of the Witwatersrand)
The current literature describes recovery from virus-induced symptoms as a RNA silencing defense, but immunity-related genes, including the structurally specific resistance gene analogs (RGAs) that may play a key role in tolerance and recovery is not yet reported. In this study, the transcriptome data of tolerant cassava TME3 (which exhibits a recovery phenotype) and susceptible cassava T200 infected with South African cassava mosaic virus were explored for RGAs. Putative resistance protein anal...
Published on Oct 1, 2015in Plant Molecular Biology3.93
Louis Bengyella1
Estimated H-index: 1
(University of the Witwatersrand),
Sayanika Devi Waikhom7
Estimated H-index: 7
(Manipur University)
+ 1 AuthorsChrissie Rey4
Estimated H-index: 4
(University of the Witwatersrand)
Plant recovery from viral infection is characterized by initial severe systemic symptoms which progressively decrease, leading to reduced symptoms or symptomless leaves at the apices. A key feature to plant recovery from invading nucleic acids such as viruses is the degree of the host’s initial basal immunity response. We review current links between RNA silencing, recovery and tolerance, and present a model in which, in addition to regulation of resistance (R) and other defence-related genes by...
Published on Apr 1, 2015in Nature Methods28.47
Daehwan Kim7
Estimated H-index: 7
(Johns Hopkins University),
Ben Langmead20
Estimated H-index: 20
(Johns Hopkins University),
Steven L. Salzberg121
Estimated H-index: 121
(Johns Hopkins University)
HISAT (hierarchical indexing for spliced alignment of transcripts) uses global and local indices for fast, sensitive alignment with small memory requirements.
Orsolya Viczián4
Estimated H-index: 4
,
András Künstler6
Estimated H-index: 6
(MTA: Hungarian Academy of Sciences)
+ 1 AuthorsLóránt Király15
Estimated H-index: 15
High concentrations of the reactive oxygen species (ROS) superoxide (O2•−) and hydrogen peroxide (H2O2) contribute to the induction of plant cell and tissue death (necrosis). In an effort to create transgenic plants with high antioxidant capacity that could resist necrotic symptoms we produced two transgenic tobacco (Nicotiana tabacum cv. SR1) lines (S1 and S2) overexpressing a tomato chloroplast superoxide dismutase (SlChSOD). SOD genes encode for antioxidant enzymes that dismutate superoxide t...
Cited By0
Newest
View next paperA common set of developmental miRNAs are upregulated in Nicotiana benthamiana by diverse begomoviruses