Pillar array columns for peptide separations in nanoscale reversed-phase chromatography
Abstract
Reversed-phase chromatography is the most common technique for separation of tryptic peptides. In this short communication, we describe the optimization of sample loading and separation parameters for a novel micromachined column and provide a detailed description on the performance and reproducibility of this separation system. Tryptic digest of a mixture of seven proteins with diverse mass and isoelectric point was used as a test sample. The...
Paper Details
Title
Pillar array columns for peptide separations in nanoscale reversed-phase chromatography
Published Date
Oct 1, 2019
Journal
Volume
1603
Pages
426 - 432
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