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Development of canine X-chromosome inactivation pattern analysis for the detection of cell clonality by incorporating the examination of the SLIT and NTRK-like family member 4 (SLITRK4) gene

Published on Jun 1, 2019in Research in Veterinary Science1.75
· DOI :10.1016/j.rvsc.2019.06.004
A. Tomita (UTokyo: University of Tokyo), H. Mochizuki (NCSU: North Carolina State University)+ 11 AuthorsHajime Tsujimoto30
Estimated H-index: 30
(UTokyo: University of Tokyo)
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Abstract
Abstract X-chromosome inactivation pattern (XCIP) analysis can be used to assess the clonality of cell populations of various origin by distinguishing the methylated X chromosome from the unmethylated X chromosome. In this study, the utility of XCIP analysis was improved by incorporating the examination of AC dinucleotide repeats in SLIT and NTRK-like family member 4 ( SLITRK4 ) gene into the previously reported CAG repeat examination of androgen receptor ( AR ) gene in dogs. The rate of heterozygosity when both genes were analysed (125/150, 83.3%) was higher than AR gene examination alone (86/150, 57.3%). Blood samples from heterozygous dogs in either AC-1 or AC-2 of SLITRK4 gene were examined for the corrected inactivation allele ratio (CIAR), resulting in the determination of a reference range of CIAR 3.8, indicating the presence of a clonal population. Through the present study, the availability of canine XCIP analysis was improved by incorporating the examination of the SLITRK4 gene, providing a highly useful laboratory examination system for the detection of the clonality of various cell/tissue samples in dogs.
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References28
Newest
Published on Jun 8, 2015in PLOS ONE2.78
Hiroyuki Mochizuki5
Estimated H-index: 5
(NCSU: North Carolina State University),
Katherine Kennedy2
Estimated H-index: 2
(NCSU: North Carolina State University)
+ 1 AuthorsMatthew Breen43
Estimated H-index: 43
Activating mutations of the BRAF gene lead to constitutive activation of the MAPK pathway. Although many human cancers carry the mutated BRAF gene, this mutation has not yet been characterized in canine cancers. As human and canine cancers share molecular abnormalities, we hypothesized that BRAF gene mutations also exist in canine cancers. To test this hypothesis, we sequenced the exon 15 of BRAF, mutation hot spot of the gene, in 667 canine primary tumors and 38 control tissues. Sequencing anal...
Published on Jan 1, 2015in Veterinary Pathology2.12
Hiroyuki Mochizuki7
Estimated H-index: 7
(UTokyo: University of Tokyo),
Yuko Goto-Koshino14
Estimated H-index: 14
(UTokyo: University of Tokyo)
+ 3 AuthorsHajime Tsujimoto23
Estimated H-index: 23
(UTokyo: University of Tokyo)
X-chromosome inactivation pattern (XCIP) analysis has been widely used to assess cell clonality in various types of human neoplasms. In this study, a polymerase chain reaction–based canine XCIP analysis of the androgen receptor (AR) gene was applied for the assessment of cell clonality in canine hematopoietic tumors. This XCIP analysis is based on the polymorphic CAG repeats in the AR gene and the difference of methylation status between active and inactive X chromosomes. We first examined the p...
Published on Aug 1, 2013in Journal of Comparative Pathology1.25
N.M. Delcour2
Estimated H-index: 2
(FU: Free University of Berlin),
Robert Klopfleisch27
Estimated H-index: 27
(FU: Free University of Berlin)
+ 1 AuthorsA.Th.A. Weiss4
Estimated H-index: 4
(FU: Free University of Berlin)
Summary Canine cutaneous histiocytoma (CCH) is the most common skin tumour of young dogs. CCH is assumed to be a tumour, but as these lesions often undergo complete regression, they have also been proposed to represent hamartomas or unregulated hyperplasia of polyclonal histiocytic cells. To demonstrate a monoclonal origin of CCH, and thereby a probable neoplastic nature, a clonality assay was established based on the heterozygosity of a polymorphic microsatellite within the canine androgen rece...
Published on Nov 1, 2012in Veterinary Pathology2.12
Hiroyuki Mochizuki7
Estimated H-index: 7
(UTokyo: University of Tokyo),
Yuko Goto-Koshino14
Estimated H-index: 14
(UTokyo: University of Tokyo)
+ 3 AuthorsHajime Tsujimoto23
Estimated H-index: 23
(UTokyo: University of Tokyo)
X-chromosome inactivation pattern (XCIP) analysis has been widely used to assess cell clonality in various types of neoplasms in humans. In the present study, a polymerase chain reaction–based feline XCIP analysis using the feline androgen receptor gene was developed. To construct the system of the analysis, polymorphism in CAG tandem repeats within the feline androgen receptor gene was explored using somatic DNAs from 50 male and 103 female cats. CAG tandem repeats in exon 1 of the feline andro...
Published on Mar 29, 2012in Blood16.56
Sabina Swierczek14
Estimated H-index: 14
(UofU: University of Utah),
Lucie Piterkova1
Estimated H-index: 1
(Palacký University, Olomouc)
+ 7 AuthorsJosef T. Prchal39
Estimated H-index: 39
(ARUP Laboratories)
Clonality can be established by a lack of mosaicism in a female because of random inactivation of either the maternal or paternal X chromosome early in embryogenesis. The methylation status of CpG sites close to the trinucleotide repeats in exon 1 of the human androgen receptor (AR) X chromosome gene assay (HUMARA) has been used to determine clonality. This HUMARA at times indicated clonal hematopoiesis in healthy elderly women, thus precluding its applicability. We used a clonality assay based ...
Published on Jan 1, 2012in Veterinary Immunology and Immunopathology1.85
Hiroyuki Mochizuki7
Estimated H-index: 7
(UTokyo: University of Tokyo),
Kenji Nakamura6
Estimated H-index: 6
(UTokyo: University of Tokyo)
+ 10 AuthorsKoichi Ohno36
Estimated H-index: 36
(UTokyo: University of Tokyo)
Abstract Lymphoid neoplasms are usually diagnosed on the basis of cytological and histopathological findings. However, in some cases, discrimination of lymphoid neoplasms from reactive lymphoid proliferation is difficult. Polymerase chain reaction (PCR) amplification of the complementarity-determining region (CDR) 3 of the T-cell receptor ( TCR ) γ gene can be used to assess clonality of T-cell populations as a supportive diagnostic tool for T-cell neoplasms. Because the length variation in the ...
Published on Sep 1, 2011in Veterinary Immunology and Immunopathology1.85
Hiroyuki Mochizuki7
Estimated H-index: 7
(UTokyo: University of Tokyo),
Kenji Nakamura6
Estimated H-index: 6
(UTokyo: University of Tokyo)
+ 8 AuthorsHiroyuki Nakayama29
Estimated H-index: 29
(UTokyo: University of Tokyo)
Abstract Lymphoid neoplasms are usually diagnosed on the basis of cytological and histopathological findings. However, in some cases, discrimination of lymphoid neoplasms from reactive lymphoid proliferation is difficult. PCR amplification of complementarity-determining region 3 (CDR3) of the immunoglobulin heavy-chain variable region ( IGHV ) gene can be used to assess clonality of B-cell populations as a supportive diagnostic tool for B-cell neoplasms. Because of the sequence variation and pos...
Published on Sep 1, 2011in The Journal of Molecular Diagnostics4.43
Birgitte Bertelsen9
Estimated H-index: 9
,
Zeynep Tümer29
Estimated H-index: 29
,
Kirstine Ravn18
Estimated H-index: 18
The analysis of X chromosome inactivation (XCI) patterns is a widely used diagnostic tool in clinical practice when investigating X-linked diseases. The most commonly used assay to determine XCI patterns takes advantage of a locus within the androgen receptor (AR) gene. This PCR-based assay relies on two differentially methylated restriction enzyme sites (HpaII) and a polymorphic repeat located within this locus. Although highly informative, this locus is not always sufficient to evaluate the X-...
Published on May 1, 2011in Experimental Hematology2.46
Stephanie Beurlet6
Estimated H-index: 6
(French Institute of Health and Medical Research),
Patricia Krief12
Estimated H-index: 12
(French Institute of Health and Medical Research)
+ 5 AuthorsBruno Cassinat32
Estimated H-index: 32
(French Institute of Health and Medical Research)
Objective Primary polycythemia in dogs is classified as a myeloproliferative syndrome with a chronic progressive course and unspecific symptoms. Diagnosis is based on exclusion criteria. In humans, the presence of an acquired recurrent mutation within the JAK2 gene has recently been identified in 90% of the patients with polycythemia vera. This mutation (V617F) is located in the pseudokinase domain of JAK2, leading to constitutive activation of the kinase responsible for the polycythemia. Detect...
Published on Sep 1, 2010in Cancer6.10
Kurt B. Hodges2
Estimated H-index: 2
(IU: Indiana University),
Oscar W. Cummings54
Estimated H-index: 54
(IU: Indiana University)
+ 6 AuthorsLiang Cheng63
Estimated H-index: 63
(IU: Indiana University)
BACKGROUND: Hepatocellular carcinoma is the most common primary tumor of the liver. Patients frequently have multiple histologically similar, but anatomically separate tumors. The clonal origin of multiple hepatocellular carcinomas is uncertain. METHODS: The authors analyzed 31 tumors from 12 different patients (11 women, 1 man), who had multiple hepatocellular carcinomas involving 1 or both lobes. Genomic DNA was extracted from formalin-fixed, paraffin-embedded tissue using laser capture microd...
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